Applied Biosystems™ Septa for 96-Well Plates, for 3500/SeqStudio™ Flex

Catalog No.	Quantity
44-126-14	20 each

Catalog No. 44-126-14 Supplier Applied Biosystems™ Supplier No. 4412614

Description

These septa for 96 well plates are designed and validated for use with Applied Biosystems 3500 and SeqStudio Flex Series Genetic Analyzers.

These septa for 96 well plates are designed and validated for use with Applied Biosystems 3500 and SeqStudio Flex series genetic analyzers.

Specifications

• Content And Storage: One bag of 20 septa. Store at room temperature.
• Format: 96-well Plate
• For Use With (Equipment): 3500 Genetic Analyzer, 3500xL Genetic Analyzer
• Product Type: Septa Plate
• Quantity: 20 each
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

How often can the plate septa on the 3130/xl, 3730/xl, 3500/xl, and SeqStudio instruments be used?

We officially recommend that the plate septa be used one time only. However, we are aware that some of our customers will rinse the septa with water and re-use them. In those instances, the septa can become sticky and gummy over time and eventually start sticking to the array. In the best case scenario, the array will lift the plate off of the autosampler just enough to break the connection with the plate sensor and abort the run. The Event Log might show that the run aborted due to the user aborting it, so the run will appear to have completed or stopped with no errors and no data. The worst case scenario is that the plate remains on the array as the autosampler starts to move, bending the electrodes and breaking the array. We recommend replacing the plate septa regularly (replacing after each run is recommended). Do not use bleach, detergents, or solvents to clean the septa, it will cause them to dry out faster and also risks contaminating the system.

When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

For Research Use Only. Not for use in diagnostic procedures.