Abnova ES-D3 (Mouse pluripotent embryonic stem cell) whole cell lysate, nondenatured

Description

• Stem cells maintained in the undifferentiated state using specialized culturing protocols
• Monitored for undifferentiated cell morphology
• Analyzed using Flow Cytometry against specific cell marker
• Coommasie Blue Staining of total cell lysate in SDS-PAGE

Western Blotting, Immunoprecipitation

Specifications

• Tissue: Embryonic
• For Use With (Application): Immunoprecipitation, Western Blot
• Description: Whole cell lysate (non-denatured)
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Quantity: 50 μg
• Storage Buffer: In modified RIPA Lysis Buffer.
• Host Species: Mouse
• Concentration: 4 mg/mL
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.