MilliporeSigma™ AbSurance™ Complete Core Histone Antibody Specificity Array

Description

Requires

Primary Antibody, Secondary Antibody, Methanol, TBST Solution, Blocking Solution (5 % non-fat dried milk in TBST), Immobilon™ Western Chemiluminescent HRP Substrate, or RapidStep™ ECL Reagent, Containers for the membrane, Shaker platform, Micropipettes and pipette tips, Film or Imager

Enables Western-blot–like detection approach using standard chemiluminescent reagents followed by image capture with either film or CCD imaging system

• Simple Western blot-like detection procedure
• 〉95 % pure peptides analyzed by mass spectrometry
• Two concentrations of peptides allows detection of both strong and weak cross-reactivity
• Consistent and uniform spotting of peptides using proprietary process
• Data collection using either film or standard CCD imaging systems
• Easy- to-read 8 x 12 macroarray
• PVDF-FL membrane allows sensitive chemiluminescent or fluorescent detection
• Positive control IgG spots and membrane landmarks for simple and easy spot identification

Specifications

• Content And Storage: Room Temperature up to 6 Months
• Detection Method: Chemiluminescence, Fluorescence
• For Use With (Application): Antibody specificity screening of histone peptides
• Quantity: 2 Arrays