Novus Biologicals™ ACE-2 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat ACE-2.

Specifications

• Assay Range: 125 pg/mL
• Assay Sensitivity: 125-8000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat ACE-2.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15
• Gene ID (Entrez): 59272
• Gene Symbol: ACE2
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 109 ng of recombinant Rat ACE-2.
  Capture Antibody: 0.5 mg/mL of mouse anti-Rat ACE-2 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-ACE-2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Immunology, Virology Bacteria and Parasites
• Storage Requirements: Storage is content dependent.
• Target: ACE-2
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.