Invitrogen™ Acetylated Lysine Monoclonal Antibody (1C6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA12021

Catalog No. MA12021

Description

MA1-2021 detects human and mouse, and monkey acetylated lysine. MA1-2021 has been used successfully in Western blot, immunoprecipitation, dot blot, ChIP Assay, and immunofluorescence (cells) procedures. By Western blot, this antibody may detect a ladder of acetylated proteins in HeLa cell lysate. In immunofluorescence procedures, MA1-2021 recognizes acetylated lysine in gamma irradiated HeLa cells. The MA1-2021 immunogen is a synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Specifications

• Antigen: Acetylated Lysine
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Peptide Array, ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1C6
• Conjugate: Unconjugated
• Gene Alias: Acetyl Lysine; Acetylated Lysine; Acetyllysine
• Immunogen: Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov