Invitrogen™ Acrosin Monoclonal Antibody (ACR-2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119180

Catalog No. 01-675-950

Description

This antibody reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa. This antibody will not cross-react with human, canine or bovine. Immunocytochemistry: Membrane permeabilization (acetone) is essential.

ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Specifications

• Antigen: Acrosin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P08001
• Gene Symbols: ACR
• Immunogen: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: ACR-2
• Conjugate: Unconjugated
• Gene: ACR
• Gene Alias: 53 kDa fucose-binding protein; Acr; Acro; Acrosin; Acrosin heavy chain; acrosin light and heavy chain prepropeptide; Acrosin light chain; acrosin precursor (EC 3.4.21.10); acrosin prepropeptide; ACRS; preproacrosin; proacrosin
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 397098
• Content And Storage: 4°C, do not freeze
• Form: Liquid