Invitrogen™ Acrosomal Vesicle Protein Monoclonal Antibody (Ds-2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119208

Catalog No. 01-675-972

Description

This antibody reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm. Immunocytochemistry: Membrane permeabilization (acetone) is essential.

This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. This gene consists of 4 exons and its alternative splicing generates multiple distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Specifications

• Antigen: Acrosomal Vesicle Protein
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: 0
• Gene Symbols: ACRV1
• Immunogen: Freshly ejaculated dog sperms.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Clone: Ds-2
• Conjugate: Unconjugated
• Gene: ACRV1
• Gene Alias: acrosomal protein SP-10; Acrosomal vesicle protein 1; Acrv1; D11S4365; Msa63; MSA-63; rSP10; Sp10; SP-10; SPACA2; sperm membrane protein; sperm protein 10
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 607249
• Content And Storage: 4°C, do not freeze
• Form: Liquid