Invitrogen™ AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™

Catalog No.	Quantity
14-651-982	100 μg

Catalog No. 14-651-982 Supplier Invitrogen™ Supplier No. 14651982

Description

Rat Monoclonal Antibody

Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2. Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence. Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 μg/mL for western blot and at less than or equal to 1.25 μg/mL for immunocytochemistry/immunofluorescence.

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or licer' activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

Specifications

• Antigen: AGO2
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: AGO2 11A9
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: AGO2
• Gene Accession No.: Q9UKV8
• Gene Alias: 1110029L17Rik; 2310051F07Rik; AGO1; AGO2; AI225898; AL022874; argonaute 2; argonaute 2, RISC catalytic component; argonaute RISC catalytic component 2; argonaute RISC catalytic subunit 2; Argonaute1; argonaute2; argonaute2 {ECO:0000255; AW546247; CTA-204B4.6; eIF2C; eIF2C 1; eIF-2C 1; eIF2C 2; eIF-2C 2; eIF2C 2 {ECO:0000255; eIF-2C 2 {ECO:0000255; EIF2C1; Eif2c2; ENSMUSG00000072493; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255; eukaryotic translation initiation factor 2C, 2; GERp95; Gm10365; Golgi ER protein 95 kDa; hAgo1; hAgo2; HAMAP-Rule:MF_03031}; Kiaa4215; mAgo2; mKIAA4215; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; protein argonaute-2; Protein slicer; Q10
• Gene Symbols: AGO2
• Host Species: Rat
• Immunogen: Peptide of human N-terminus.
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 27161
• Target Species: Human
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ