Invitrogen™ AHR Monoclonal Antibody (RPT9)

Catalog No.	Quantity
MA1513	100 μL

Catalog No. MA1513 Supplier Invitrogen™ Supplier No. MA1513

Description

Mouse Monoclonal Antibody

MA1-513 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells. MA1-513 has been successfully used in immunoprecipitation, chromatin immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence, and gel shift procedures. This antibody specifically immunoprecipitates a single ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-513 can be used in Western blotting procedures, MA1-514 (clone RPT1) is recommended for this procedure. The MA1-513 immunogen is a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

Specifications

• Antigen: AHR
• Applications: ChIP Assay, Flow Cytometry, Gel Shift, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: RPT9
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: AHR
• Gene Accession No.: P30561, P35869, P41738
• Gene Alias: Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In
• Gene Symbols: AHR
• Host Species: Mouse
• Immunogen: Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11622, 196, 25690
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG3 κ