Invitrogen™ Multispecies AKT1 (Total) InstantOne™ ELISA Kit

Catalog No.	Quantity
50-112-8670	96 Tests

Catalog No. 50-112-8670 Supplier Invitrogen™ Supplier No. 858604711

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total human, mouse, and hamster AKT 1 in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Specifications

• Accession Number: P31749
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human, Rat, Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 207
• Gene Symbol: AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Protein kinase B, PKB, RAC-alpha serine/threonine-protein kinase
• Target: AKT1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 10 mins