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Invitrogen™ AKT1 (Total) Multispecies InstantOne™ ELISA Kit Non-distribution product as customer accommodation.

InstantOne ELISA

Manufacturer:  Invitrogen™ 858604711

Catalog No. 50-112-8670


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Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
AKT 1 (Total) Capture Antibody Reagent
AKT 1 (Total) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal

Description

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of total human, mouse, and hamster AKT 1 in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Specifications

Specifications

P31749
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Cell Lysate,20 μL
2-8°C
1 hr 10 min
AKT1
HRP
Biotin
207
Capture Antibody
96 tests
Cell Lysate
Human, Mouse, Rat
1 hr 20 min
Human
Protein kinase B, PKB, RAC-alpha serine/threonine-protein kinase
Documents
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