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Invitrogen™ Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit

Catalog No. 501129232
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96 Tests
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50-112-9232 96 Tests
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Catalog No. 50-112-9232 Supplier Invitrogen™ Supplier No. 858604611
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Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
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Specifications

Accession Number P31749,P31751,Q9Y243
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Mouse, Human, Rat
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 10000, 207, 208
Gene Symbol AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-Pk.-gamma,RAC-gamma,STK-2
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym Protein Kinase B, PKB, RAC-alpha serine/threonine-protein kinase
Target AKT Pan
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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