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Invitrogen™ AKT (Total/Phospho) Multispecies InstantOne™ ELISA Kit Non-distribution product as customer accommodation.

InstantOne ELISA

Manufacturer:  Invitrogen™ 858604611

Catalog No. 50-112-9232


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Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
AKT 1 (Total) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
AKT 1 (Total) Detection Antibody Reagent
phospho-AKT (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal

Description

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

AKT (protein kinase B, PKB, RAS-alpha) is a 57kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. AKT is activated by insulin, PI3K, IGF1 and various other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. The AKT pathway is a major target for cancer therpeutics where AKT signaling dysfunction has implicated in many types of cancer, including cancer syndromes known as phakomatoses.
Specifications

Specifications

P31749,P31751,Q9Y243
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Cell Lysate,20 μL
2-8°C
1 hr 10 min
AKT1, AKT2, AKT3
HRP
Biotin
10000, 207, 208
Capture Antibody
96 tests
Cell Lysate
Human, Mouse, Rat
1 hr 20 min
Human
Protein Kinase B, PKB, RAC-alpha serine/threonine-protein kinase
Documents
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