Thermo Scientific Chemicals Alkaline Phosphatase, calf intestine, EIA Grade

Catalog No.	Quantity
AAJ61037LB0	5mg
AAJ61037MCR	1mg

Catalog No. AAJ61037LB0 Supplier Thermo Scientific Chemicals Supplier No. J61037LB0

Description

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Specifications

Chemical Identifiers

• CAS: 9001-78-9

• Content And Storage: Store at -20°C
• Format: Liquid, 140 kDa
• Molecular Weight (g/mol): 496.569
• Product Type: Alkaline Phosphatase
• Quantity: 5mg

Frequently Asked Questions (FAQs)

What are the applications of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

BioAcryl-P (30%, 37.5:1) is suitable for conjugation to antibodies and other proteins for ELISA, Western blotting, and other histochemical procedures. Alkaline phosphatase may be used to dephosphorylate the 5' ends of DNA or RNA. It can also be used to dephosphorylate proteins.

What is the specific activity of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The specific activity of BioAcryl-P (30%, 37.5:1) is ≥3,000 units/mg protein (DEA units).

What is the enzyme affinity (Km) of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The Km is 1.5 x 10^-3 M (p-Nitrophenyl phosphate); 19 x 10^-3 M (phosphoenolypyruvate).

What is the extinction coefficient of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The extinction coefficient (278nm) of BioAcryl-P (30%, 37.5:1) is 7.6-10.5.

What is the molecular mass of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The molecular mass of BioAcryl-P (30%, 37.5:1) is ~ 140 kDa.

What is the enzyme commission number (EC) of BioAcryl-P (30%, 37.5:1) (Cat. No. J61505.K2, J61505-K2)?

The EC for BioAcryl-P (30%, 37.5:1) is 3.1.3.1.

What is the definition of one unit of Alkaline Phosphatase, calf intestine, EIA Grade (Cat. Nos. J61037.xx)?

One unit is defined as the amount of protein that hydrolyzes 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C. One glycine unit is equal to ~3 DEA units.

What is the optimum pH for Alkaline Phosphatase, calf intestine, EIA Grade (Cat. Nos. J61037.xx)?

Optimum pH varies depending on the substrate, substrate concentration, and the ionic concentration. The enzyme is most stable at a pH range of 7.5-9.5. The optimum pH range for catalytic activity is 8-10. The enzyme has optimal activity using Diethanolamine (DEA) as a substrate at a pH of ~9.8.

How can I inhibit Alkaline Phosphatase enzyme activity for Alkaline Phosphatase, calf intestine, EIA Grade (Cat. Nos. J61037.xx)?

The enzyme activity can be inhibited by addition of chelating agents or inorganic phosphates.

What are the concentrations of the components in Alkaline Phosphatase, calf intestine, EIA Grade (Cat. Nos. J61037.xx)?

Alkaline Phosphatase, calf intestine, EIA Grade is supplied as a 20 mg/mL protein solution in 50% glycerol, containing 5 mM Tris, 5 mM MgCl2 and 0.12 mM ZnCl2.

What storage conditions should I use for Alkaline Phosphatase, calf intestine, EIA Grade (Cat. Nos. J61037.xx)?

Alkaline Phosphatase, calf intestine, EIA Grade should be stored at -20°C.

Safety and Handling

SDS

RUO – Research Use Only