Cytiva AlkPhos Direct™ Hybridization Buffer for Direct Labeling System

Description

Includes

Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 5000cm² membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star™ or ECF detection reagents.

Fast and convenient system for all high-sensitivity applications using DNA, RNA, or oligonucleotide probes

• Hybridization stringency can be controlled by elevating temperatures up to 75°C (167°F), or by varying salt concentration
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• ECF™ detection for quantitation experiments
• Thirty minutes from completion of hybridization to application of detection reagent: saves two to three hours over conventional indirect methods
• Resulting probes can be used without purification

Specifications

• For Use With (Application): Fast, convenient for high-sensitivity applications with DNA, RNA, Oligo probes
• Includes: Labeling reagent, crosslinker, control DNA, Reaction Buffer, hybridization Buffer, blocking agent, CDP-Star™ or ECF detection reagents
• Product Type: Amersham AlkPhos Direct Hybridization Buffer sufficient for 5000 cm²
• Quantity: For 5000 cm2 membrane