Invitrogen™ alpha-Fetoprotein Monoclonal Antibody (1E8), Biotin, eBioscience™

Catalog No.	Quantity
13-949-982	100 μg

Catalog No. 13-949-982 Supplier Invitrogen™ Supplier No. 13949982

Description

Mouse Monoclonal Antibody

This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions. Applications Reported: This 1E8 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 1E8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high pH antigen retrieval at 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.

Specifications

• Antigen: alpha-Fetoprotein
• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: 1E8
• Concentration: 0.5 mg/mL
• Conjugate: Biotin
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: AFP
• Gene Accession No.: P02771
• Gene Alias: a fetoglobulin; a fetoprotein; Afp; AFPD; Alpha fetoglobulin; alpha fetoprotein; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-fetoprotein; alpha-fetoprotein precursor; alpha-foetoprotein; chloride intracellular channel 6; FETA; fetoglobulin; fetoprotein; HPAFP; α fetoglobulin; α fetoprotein
• Gene Symbols: AFP
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 174
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ