Invitrogen™ alpha Internexin Monoclonal Antibody (2E3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 323600

Catalog No. 32-360-0

Description

This antibody is purified from mouse ascites fluid and is specific for the 66 kDa alpha-internexin protein. 2E3 antibody has stained human neuron and nerve fiber in human cerebral cortex. It has also stained glioma, ganglioneuroma, pheochromocytoma, and paraganglioma in human tissue. It is recommended that FFPE tissues are pretreated with HIER using citrate buffer, pH 6.

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Specifications

• Antigen: alpha Internexin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: P23565, P46660, Q16352
• Gene Symbols: INA
• Immunogen: Full-length recombinant rat alpha-internexin protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: 2E3
• Conjugate: Unconjugated
• Gene: INA
• Gene Alias: 66 kDa neurofilament protein; Alpha-internexin; alpha-Inx; AV028420; FLJ18662; FLJ57501; Ina; Inexa; internexin neuronal intermediate filament protein alpha; internexin neuronal intermediate filament protein, alpha; internexin, alpha; Intlaa; LOW QUALITY PROTEIN: alpha-internexin; MGC12702; NEF5; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; Nf66; NF-66; TXBP-1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100348512, 226180, 24503, 9118
• Content And Storage: -20°C
• Form: Liquid