Invitrogen™ alpha Synuclein Monoclonal Antibody (4F1), APC

Catalog No.	Quantity
PIMA545846	100 μg

Catalog No. PIMA545846

Supplier: Invitrogen™ MA545846

Description

Mouse Monoclonal Antibody

A 1:1,000 dilution of MA5-45846 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Binds multiple linear epitopes within human alpha synuclein: 21-70 aa, 61-90 aa, and 101-140 aa, determined using Dot blot and ELISA with corresponding peptides. Detects approximately 14 kDa band corresponding to Alpha synuclein. Band at approximately 40 kDa is a trimer.

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

Specifications

• Antigen: alpha Synuclein
• Applications: Dot Blot, ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 4F1
• Concentration: 1 mg/mL
• Conjugate: APC
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene: SNCA
• Gene Accession No.: O55042, P37377, P37840
• Gene Alias: Alpha synuclein; alphaSYN; alpha-synuclein; NACP; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; Snca; Syn; synuclein alpha; synuclein alpha-140; synuclein, alpha; synuclein, alpha (non A4 component of amyloid precursor)
• Gene Symbols: SNCA
• Host Species: Mouse
• Immunogen: Mouse alpha synuclein aggregate.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20617, 29219, 6622
• Target Species: Human, Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1