Learn More
Invitrogen™ Ambion™ DNase I (RNase-free)
Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin
Manufacturer: Invitrogen™ AM2222
Description
DNase I functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with 5'-phosphate and 3'-hydroxyl group. RNase-free DNase I is recommended to degrade DNA in presence of RNA, in absence of RNase is critical to maintain integrity of RNA. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells that may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR.
- Tested for contaminating RNase and protease activity
- Functionality is determined by digestion of human genomic DNA followed by quantitative real-time PCR to detect undigested DNA
- Requires bivalent cations (Mg2+ and Ca2+ at approximately 5mM and 0.5mM, respectively) for maximal activity, and has a pH optimum of 7.8
- Unit definition: One unit is amount of enzyme required to completely degrade 1μg DNA in 10 min. at 37°C, and is equivalent to 0.04 Kunitz units
For an alternative to bovine DNase I, please consider Recombinant DNase I (Mfr. No. AM2235). For a more active, salt-tolerant DNase, please see the TURBO™ DNase products (Mfr. Nos. AM2239 and AM2238).
PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
Specifications
| -20°C | |
| DNase | |
| DNase I |
| Dry Ice | |
| 2000 U | |
| Ambion™ |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.