Invitrogen™ Poly(A) Polymerase (cloned) 2 U/μL
Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion
Supplier: Invitrogen™ AM2030
Includes: Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25mM MnCl2
- The Invitrogen Poly(A) Polymerase enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs
- One tube containing 80U (2U/μL), 5X Poly(A) Polymerase Buffer, and 25mM MnCl2 are provided
- The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence
- Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of AMP into acid-insoluble material in 10 minutes at 37°C
DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, PCR & Real-Time PCR, RNA Labeling, Reverse Transcription
Shipping Condition: Dry ice
|Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.|
|Poly(A) RNA Polymerases|
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For Research Use Only. Not for use in diagnostic procedures.