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Invitrogen™ Poly(A) Polymerase (cloned) 2 U/μL

Catalog No. AM2030
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Quantity:
80 units, 2 μL

Description

Includes

Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25mM MnCl2

Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion

  • The Invitrogen Poly(A) Polymerase enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs
  • One tube containing 80U (2U/μL), 5X Poly(A) Polymerase Buffer, and 25mM MnCl2 are provided
  • The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence
  • Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of AMP into acid-insoluble material in 10 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, PCR & Real-Time PCR, RNA Labeling, Reverse Transcription

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.
Shipping Condition Dry Ice
Polymerase Poly(A) RNA Polymerase
Product Line Ambion™
Quantity 80 units, 2 μL
Concentration 5X
Source RNA

For Research Use Only. Not for use in diagnostic procedures.

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