Invitrogen™ Poly(A) Polymerase (cloned) 2 U/μL

Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion

Supplier:  Invitrogen™ AM2030

Catalog No. AM2030

Includes: Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25mM MnCl₂

Description

• The Invitrogen Poly(A) Polymerase enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs
• One tube containing 80U (2U/μL), 5X Poly(A) Polymerase Buffer, and 25mM MnCl₂ are provided
• The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence
• Unit Definition: One unit is the amount of enzyme required to incorporate 1nmol of AMP into acid-insoluble material in 10 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, PCR & Real-Time PCR, RNA Labeling, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl₂ should be stored at –20°C.
• Polymerase: Poly(A) RNA Polymerase
• Quantity: 2 μL
• Source: RNA

• Shipping Condition: Dry Ice
• Product Line: Ambion™
• Concentration: 5X

For Research Use Only. Not for use in diagnostic procedures.