Invitrogen™ SYBR™ Green Cells-to-C_T™ Control Kit

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-C_T™ Kits with sufficient reagents are provided for 100 reactions.

Supplier:  Invitrogen™ 4402959

Catalog No. 44-029-59

Includes: 100μL Xeno RNA Control, 10⁵ copies/μL; 250μL 20X SYBR Xeno Primers; 250μL 20X SYBR ACTB Primers; Amounts: Xeno RNA Control: approximately 100 lysis reactions, SYBR Xeno and SYBR ACTB Primers: up to 250 PCRs (20μL) each

Description

This kit is designed for use with the Power SYBRGreen (Cat. No. 4402953, 4402954, and 4402955) and Fast SYBRGreen Cells-to-C_T™ Kits (Cat. No. 4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBRGreen-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBRACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.

• Polymerase: AmpliTaq Gold™ DNA Polymerase
• Purity/Quality Grade: UP (Ultra Pure)

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  Components:
  • 100 μL Xeno™ RNA Control, 10⁵ copies/μL
  • 250 μL 20X SYBR Xeno Primers
  • 250 μL 20X SYBR ACTB Primers
  
  Amounts:
  • Xeno RNA Control: approximately 100 lysis reactions
  • SYBR Xeno and SYBR ACTB Primers: up to 250 x 20 μL PCRs
  
  Storage Conditions: Store at −20°C. Do not store in a frost-free freezer.

• Format: Tube
• Green Features: Fewer resources used and less waste, Less hazardous
• Product Line: Cells-to-CT™, SYBR™
• Shipping Condition: Dry Ice
• No. of Reactions: 100 Reactions

• Control Template: XenoRNA™ control (synthetic RNA transcript)
• Gene Symbol: ACTB
• Label or Dye: Unconjugated
• Quantity: 100 Reactions
• Target: XenoRNA™ control, β-actin

For Research Use Only. Not for use in diagnostic procedures.