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Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
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Catalog No. 4402959
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100 Reactions
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Catalog No. Quantity
44-029-59 100 Reactions
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Catalog No. 44-029-59 Supplier Invitrogen™ Supplier No. 4402959
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Description

Includes

100μL Xeno RNA Control, 105 copies/μL; 250μL 20X SYBR Xeno Primers; 250μL 20X SYBR ACTB Primers; Amounts: Xeno RNA Control: approximately 100 lysis reactions, SYBR Xeno and SYBR ACTB Primers: up to 250 PCRs (20μL) each

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

This kit is designed for use with the Power SYBRGreen (Cat. No. 4402953, 4402954, and 4402955) and Fast SYBRGreen Cells-to-CT™ Kits (Cat. No. 4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBRGreen-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBRACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.

  • Polymerase: AmpliTaq Gold™ DNA Polymerase
  • Purity/Quality Grade: UP (Ultra Pure)

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage

Components:
• 100 μL Xeno™ RNA Control, 105 copies/μL
• 250 μL 20X SYBR Xeno Primers
• 250 μL 20X SYBR ACTB Primers

Amounts:
• Xeno RNA Control: approximately 100 lysis reactions
• SYBR Xeno and SYBR ACTB Primers: up to 250 x 20 μL PCRs

Storage Conditions: Store at −20°C. Do not store in a frost-free freezer.
Control Template XenoRNA™ control (synthetic RNA transcript)
Format Tube
Gene Symbol ACTB
Label or Dye Unconjugated
Product Line Cells-to-CT, SYBR
Quantity 100 Reactions
Shipping Condition Dry Ice
Target XenoRNA™ control, β-actin
No. of Reactions 100 Reactions

For Research Use Only. Not for use in diagnostic procedures.

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