Invitrogen™ TaqMan™ Fast Cells-to-C_T™ Kit

The TaqMan™ Fast Cells-to-C_T™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

Supplier:  Invitrogen™ 4399003

Catalog No. 43-990-03

Includes: Stop Solution: 500μL tube; DNase I: 55μL tube; 20X RT Enzyme Mix: 275μL tube; Lysis Solution: 5.5mL bottle; 2X RT Buffer: 5.5mL bottle; TaqMan Fast Universal PCR Master Mix: 4 x 1.25mL bottle

Description

• Complete: Optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan™ Fast Universal PCR Master Mix
• Fast: Prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan™ Fast Universal PCR Master Mix delivers real-time PCR results in ∼35 minutes
• Convenient: Lyse samples in tube or directly in 96- or 384-well culture plates
• Robust: Perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
• Efficient: Contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  Contains:
  • Stop Solution: 500 μL tube
  • DNase I: 55 μL tube
  • 20X RT Enzyme Mix: 275 μL tube
  • Lysis Solution: 5.5 mL bottle
  • 2X RT Buffer: 5.5 mL bottle
  • TaqMan™ Fast Universal PCR Master Mix: 4 x 1.25 mL bottle
  
  Store Stop Solution, DNase I, and 20X RT Enzyme Mix at -20°C.
  Store Lysis Solution, 2X RT Buffer, and TaqMan™ Fast Universal PCR Master Mix at 4°C.

• Format: Tube
• PCR Method: 2-step RT-qPCR
• Reaction Speed: Fast
• Green Features: Fewer resources used and less waste, Less hazardous
• Product Line: Ambion™, Cells-to-CT™, TaqMan™
• Quantity: 100 reactions
• Shipping Condition: Dry Ice
• For Use With (Application): Gene Expression
• Sample Type: Cell

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Polymerase: DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Fast Cells-to-C Kit
• Reverse Transcriptase: M-MLV
• Sufficient For: 100 Reactions
• No. of Reactions: 100 reactions

For Research Use Only. Not for use in diagnostic procedures.