Invitrogen™ TURBO DNase™ (2 U/μL)

Catalog No.	Quantity
AM2238	1000 U
AM2239	5000 U

Catalog No. AM2238 Supplier Invitrogen™ Supplier No. AM2238

Description

Efficiently degrades and digests DNA

Cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides with an increased affinity for DNA-binding and remains active in presence of salt.

• Up to 50X more activity and 350% greater catalytic efficiency
• Efficiently degrades DNA in solutions containing up to 0.25M salt
• Efficiently digests DNA to oligonucleotides
• Used in clearing DNA templates from in vitro transcription reactions
• RNase-free and recombinant in origin

• Used to clear DNA contamination from RNA samples prior to RT-PCR
• Conventional DNase I has a poor affinity for DNA and cleaves DNA of low concentration
• Salt-sensitive; 20 mM NaCl can reduce activity of enzyme by 30%
• Purified from bovine pancreas, one of richest natural sources of RNase A
• Threat of contaminating RNase activity in DNase I preparations requires that enzyme be exhaustively purified

TURBO DNA-free (Mfr. No. AM1907) is available. It contains TURBO DNase along with reagents to inactivate the enzyme and remove divalent cations from the sample post-digestion.

PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Order Info

Shipping Conditions: Dry ice

Specifications

• Concentration: 2 U/μL
• Content And Storage: TURBO™ DNase and 10X Reaction Buffer should be stored at –20°C.
• Shipping Condition: Dry Ice
• Enzyme: DNase
• Compatible Buffer: 10X Reaction Buffer
• Quantity: 1000 U
• Product Type: DNase
• Product Line: Ambion, TURBO

Frequently Asked Questions (FAQs)

How do I remove DNase from an RNA sample?

DNase can be removed by the following methods: acid phenol:choloform extraction, lithium chloride precipitation, EDTA, and heat inactivation, or using our MegaClear Transcription Clean-Up Kit (Cat. No. AM1908).

Alternatively, our Invitrogen TURBO DNA-free Kit (Cat. No. AM1907) is supplied with a DNase inactivation reagent that can be used to remove the DNase, as well as divalent cations such as magnesium and calcium, which can catalyze RNA degradation when RNA is heated with the sample.

How can I inactivate DNase I?

Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.

Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.

DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.

Are your DNase I products RNase-free?

Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.

What are the ideal storage conditions for the TURBO DNase (2 U/μl) (Cat. No. AM2239, AM2238)? Will the enzyme remain stable even after multiple freeze/thaw cycles?

The TURBO DNAse itself is contained in a buffer containing glycerol, and therefore it will not freeze at -20°C. The reaction buffer is an aqueous salt solution which will remain stable even after multiple freeze/thaw cycles. We do not recommend storing it under different conditions. You could also aliquot the DNAse, but under these circumstances it wouldn't be necessary.

Does TURBO DNase contain DTT?

TURBO DNase does not contain DTT.

Can I purchase the 10X Reaction Buffer included with TURBO DNase as a stand-alone product?

We do not offer the 10X Reaction Buffer that comes with TURBO DNase as a stand-alone item and the composition of the buffer is proprietary.

Does TURBO DNase cleave both single- and double-stranded DNA?

Yes, since the cleavage site for TURBO DNase is the phosphodiester bond, it cleaves both single- and double-stranded DNA.

How does the dsDNase in Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase (Cat. Nos. K1671 and K1672) compare with TURBO DNase?

The main difference between the two enzymes is that dsDNase is heat-labile (easily inactivated by moderate heat treatment at 55 degrees C) and specific to dsDNA. Therefore, it does not need a separate heat inactivation step. It gets inactivated during the reverse transcription reaction. Turbo DNase is not heat-labile and is able to degrade both ssDNA and dsDNA. It needs to be heat inactivated post-digestion or has to be removed by phenol-chloroform extraction.

I want to use the MagMAX-96 for Microarrays Total RNA Isolation Kit to isolate total RNA with the modified spin protocol (https://bit.ly/2Z9cPQK). Can I add the TURBO DNase steps from the standard no-spin protocol to the modified spin protocol?

When using the MagMAX-96 for Microarrays Total RNA Isolation Kit modified spin protocol (https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/isolating-small-rnaas-using-the-magmax-96-for-microarrays-kit.html) for isolating large + small RNA, we recommend going through the RNA isolation procedure and then treating the eluted RNA with TURBO DNase.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.