Specifically formulated for use with oligonucleotide probes in northern analysis
Manufacturer: Invitrogen™ AM8663
- Contains a proprietary blend of hybridization accelerators and blocking agents to enhance the levels of hybridization
- Supplied in one bottle containing 125mL
- Use less probe per blot
- Decrease hybridization time to 2 hours
- Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19)
- With ULTRAhyb™ Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
- It works well with oligonucleotides having Tms from 78° to 90°C and 47 to 62% GC contents; However, the optimal hybridization temperature for oligonucleotide probes with different GC content and Tm values should be determined empirically
DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting
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Shipping Condition: Room Temperature
|Store at 4°C.|
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For Research Use Only. Not for use in diagnostic procedures.