Cytiva Amersham™ AlkPhos Direct™ ECF Detection Module

Description

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• ECF detection for quantitation experiments
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm²: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.

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Specifications

• Sufficient For: 2500cm2 membrane
• Includes: Sufficient for 25 or 50 labeling reactions and detection of 2500cm² membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
• Quantity: Each