Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Description

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.

DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm² or 5000cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

Specifications

• Content And Storage: 2°C to 8°C
• Description: AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.
• For Use With (Application): Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
• Includes: Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
• Product Type: AlkPhos Direct Labeling Module
• Quantity: 2500 cm2 Membrane