Cytiva Amersham™ ECF™ Substrate for Western Blotting

Description

Includes

ECF Substrate, 60mL; Dilution buffer

Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

• For use with fluorescence scanning equipment
• Sensitivity comparable to chemiluminescence
• Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
• Eliminate need for darkroom and film

Western blotting, dot, and slot blotting

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Specifications

• For Use With (Application): Fluorescence scanning equipment
• Includes: ECF Substrate, 60mL, Dilution Buffer
• Quantity: Sufficient reagents for detecting 2500cm² membrane