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Cytiva Amersham™ ECF™ Substrate for Western Blotting
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Catalog No. 45000947 Shop All Cytiva Products
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Sufficient reagents for detecting 2500cm2 membrane
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45-000-947 Sufficient reagents for detecting 2500cm2 membrane
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Catalog No. 45-000-947 Supplier Cytiva Supplier No. RPN5785
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Description

Includes

ECF Substrate, 60mL; Dilution buffer

Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

  • For use with fluorescence scanning equipment
  • Sensitivity comparable to chemiluminescence
  • Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminate need for darkroom and film

Western blotting, dot, and slot blotting

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Specifications

For Use With (Application) Fluorescence scanning equipment
Includes ECF Substrate, 60mL, Dilution Buffer
Quantity Sufficient reagents for detecting 2500cm2 membrane
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