Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

Catalog No.	Quantity
N8080241	250 Units
N8080247	1,000 units
N8080259	25 x 1,000 units
N8080245	3,000 units
N8080249	5,000 units

Catalog No. N8080241 Supplier Applied Biosystems™ Supplier No. N8080241

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

AmpliTaq Gold DNA Polymerase with Buffer II and MgCl₂ is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.

Features

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer II with MgCl₂
• Hot-start technology reduces risk of biological contamination

Notes

• For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
• When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL
  • 10X PCR Buffer II, 1 x 1.5 mL
  • 25 mM MgCl₂, 1 x 1.5 mL
  
  Store at -15°C to -30°C.
• Format: Tube
• GC-Rich PCR Performance: Low
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Quantity: 250 Units
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• Hot Start: Built-In Hot Start
• No. of Reactions: 200 Reactions
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 5 kb or less
• Starting Material: DNA

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.