MilliporeSigma™ Annexin A3, Mouse, Unlabeled, Clone: 9996-1-3M1/3L7-5,

Catalog No.	Quantity	Conjugate
MABS1329	100 μL	Unconjugated

Catalog No. MABS1329 Supplier MilliporeSigma™ Supplier No. MABS1329

Description

Mouse Monoclonal Antibody

Annexin A3 (UniProt P12429; also known as 35-alpha calcimedin, Annexin III, Annexin-3, Inositol 1,2-cyclic phosphate 2-phosphohydrolase, Lipocortin III, PAP-III, Placental anticoagulant protein III) is encoded by the ANXA3 (also known as ANX3) gene (Gene ID 306) in human. Annexin A3 is a Ca2+-dependent phospholipid-binding protein shown to promote angiogenesis and rat liver regeneration. Upregulated annexin A3 expression is reported in ovarian, breast, colon, lung, gastric, gallbladder, testicular, and urothelial cancers, where it is involved in promoting tumorigenesis and resistance to chemotherapy. ANXA3 is reported to represent the most significantly upregulated gene that encodes for a secretory protein in the CD133+ liver cancer stem cell (CSC) subset. Both cellular and secretory annexin A3 play pivotal roles in promoting cancer and stem cell-like features in CD133+ liver CSCs through a dysregulated JNK pathway. Annexin A3 serum levels in hepatocellular carcinoma (HCC) patients are closely associated with aggressive clinical features. Annexin A3 neutralizing antibody is shown to cause a significant reduction in HCC tumor growth and self-renewal by effectively killing off CD133+ CSCs both in cultures in vitro and in animal xenograft in vivo.

Specifications

• Antigen: Annexin A3
• Applications: Neutralization, Western Blot
• Classification: Monoclonal
• Clone: 9996-1-3M1/3L7-5
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse IgG1 in Tris-buffered saline (TBS) containing 50mM tris-HCl (pH7.5) and 150mM NaCl without preservatives.
• Gene: ANXA3, ANX3
• Gene Accession No.: P12429
• Host Species: Mouse
• Immunogen: Recombinant human annexin A3.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005130
• Test Specificity: This monoclonal antibody detected a single ∽33 kDa target band among a panel of human hepatocellular carcinoma (HCC) cell lines. The epitope has been mapped to a 10-a.a. region within the third annexin domain (Tong, M., et al. (2015). Stem Cell Reports. 5(1):45-59).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.