Invitrogen™ Annexin V Recombinant Protein, FITC, eBioscience™

Catalog No.	Quantity
50-929-0	300 Tests
50-928-9	20 Tests
50-928-8	100 Tests

Catalog No. 50-929-0 Supplier Invitrogen™ Supplier No. BMS306FI300

Description

Recombinant Protein

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Specifications

• Accession Number: P08758, P14668, P48036
• Quantity: 300 Tests
• Concentration: 5 μL/Test
• For Use With (Application): Neutralization, Flow Cytometry
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.4
• Gene ID (Entrez): 11747, 25673, 308
• Molecular Weight (g/mol): 35.8kDa
• Name: Annexin V
• pH Range: 7.4
• Purification Method: Purified
• Source: E. coli
• Storage Requirements: 4°C, store in dark, DO NOT FREEZE!
• Regulatory Status: RUO
• Structural Form: E. Coli
• Gene Alias: Anchorin CII; annexin 5; annexin A5; Annexin V; annexin-5; Anx5; ANXA5; Calphobindin I; CBP-I; Endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; LC5; lipocortin V; PAP-I; placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; R74653; RPRGL3; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha
• Common Name: Annexin V
• Gene Symbol: Anxa5
• Biological Activity: Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
• Product Type: Protein
• Specific Reactivity: All
• Conjugate: FITC
• Cross Reactivity: All
• Species: E. coli
• Recombinant: Recombinant
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >89% by SDS-PAGE and RP-HPLC

Frequently Asked Questions (FAQs)

Why is the recommended cell concentration for a test, for the Annexin V in the Annexin V-FITC Apoptosis Detection Kit, lower than the recommended 1x10^6 cells in 100uL for the Annexin V Recombinant Protein FITC?

The Annexin V-FITC Apoptosis Detection Kit has a different protocol because it has been validated in-house with that protocol and as a kit. The protocol, for the Annexin V Recombinant Protein FITC, is a generic staining protocol that generally requires optimization per application. Essentially the ideal titration would need to be determined empirically and specifically for your instruments.

The Annexin V-FITC Apoptosis Detection Kit protocol is more scrutinized and validated to work as an indicator of apoptosis, whereas the Annexin V Recombinant Protein FITC by itself is validated to bind to phosphatidylserine on cells. The standalone product allows users to co-stain with non-PI counterstains, or use it in other applications aside from traditional apoptosis.

What is the recommended cell concentration for a sample/test for the Annexin V Recombinant Protein FITC?

For this product, a test is defined as 1x10^6 cells (1million) in 100 µl of staining volume, so specifically 5 µl of the Annexin V FITC dye is enough to stain 1x10^6 cells in 100 µl.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov