Cadherin-5 Mouse anti-Human, Unlabeled, Clone: 75, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB610251	BD Biosciences 610251	50 μg
BDB610252	BD Biosciences 610252	150 μg

Description

Cadherins are a family of transmembrane glycoproteins involved in the Ca²⁺- dependent cell-cell adhesion that occurs in many tissues. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca²⁺ and protease-sensitivity, and molecular weight. Cadherin-5 (VE-Cadherin or CD144) is one of a number of cadherins (cadherin-4 through -11) whose cDNAs were isolated from rat brain and retina. These cadherins have a cytoplasmic domain that is highly conserved relative to previously identified cadherins, indicating that this domain is essential for cell adhesion activity. This function is mediated by cadherin interaction with cytoskeletal proteins. However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins. Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells. A new type of adhering junction has been identified in certain vascular endothelial cells. These junctions are known as “complexus adherens” and are morphologically and compositionally distinct from desmosomes and zonula adherens junctions. The complexus adherens of endothelial cells lack desmosomal cadherins as well as E-Cadherin. However, these cells are rich in Cadherin-5 which colocalizes with desmoplakin and γ-Catenin (plakoglobin).

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Cadherin-5
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified

• Clone: 75
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.