CD121a Rat anti-Mouse, Brilliant Violet 421, Clone: 35F5, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 564387
The 35F5 monoclonal antibody specifically binds to the Type I Interleukin-1 Receptor (IL-1 RI) which is also known as CD121a. CD121a is expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80 kDa type-I transmembrane glycoprotein, a member of the Ig superfamily that mediates the inflammatory responses of leuc ocytes to IL-1. The 35F5 antibody is capable of blocking the binding of IL-1α, IL-1β, a nd IL-1 receptor antagonist to the Type I IL-1 Receptor. The 35F5 mAb is highly specific for the Type I IL-1 Receptor and does not react with the Type II IL-1 Receptor (CD121b) which is present on mouse macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|Brilliant Violet 421|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Il1r1; IL-1 Receptor Type I/p80; IL-1 R alpha; IL-1 Rα; IL-1 RI|
|IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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