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Description
The M φP9 monoclonal antibody specifically binds to human CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, this CD14-specific antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. The purified antibody is suitable for staining acetone-fixed, frozen tissue sections.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
Specifications
| Antigen | CD14 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | MPHIP9 |
| Conjugate | Brilliant Violet 711 |
| Description | LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein |
| Formulation | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Human Monocytes |
| Purification Method | Affinity Purified |
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Safety and Handling
For Research Use Only.
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