CD16 Mouse anti-Human, Brilliant Violet 786, Clone: 3G8, BD

Catalog No.	Quantity
BDB563690	100 Tests
BDB563689	25 Tests

Catalog No. BDB563690 Supplier BD Biosciences Supplier No. 563690

Description

Mouse Monoclonal Antibody

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γRIIa and Fc γRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).

Specifications

• Antigen: CD16
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 3G8
• Conjugate: Brilliant Violet 786
• Description: FcRIII; Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; IGFR3
• Host Species: Mouse
• Immunogen: Human polymorphonuclear leukocytes
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.