CD20 Mouse anti-Human, PE-CF594, Clone: H1, BD

Catalog No.	Quantity
BDB563430	50 Tests

Catalog No. BDB563430 Supplier BD Biosciences Supplier No. 563430

Description

Mouse Monoclonal Antibody

The H1 (FB1) antibody specificially binds to a cytoplasmic domain of CD20. CD20 is a 33-37-kDa four transmembrane phosphoprotein that is expressed by B lymphocytes from the pre-B stage and most malignant B cells and is lost during plasma cell differentiation. Low level CD20 expression is observed on a subset of normal circulating T lymphocytes, and CD20-positive T-cell lymphomas have been reported. The CD20 molecule is associated with membrane lipid raft domains, acts as a channel for calcium ions, and is involved in the regulation of B cell activation and survival. The cytoplasmic domain regions are serine and threonine rich and contain multiple phosphorylation consensus sequences.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Specifications

• Antigen: CD20
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: H1
• Conjugate: PE-CF594
• Description: MS4A1; membrane-spanning 4-domains subfamily A member 1; B1; Bp35; LEU-16
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human B lymphoma cell line
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ