CD3 Mouse anti-Rat, Brilliant Violet 421, Clone: 1F4, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563948

Catalog No. BDB563948

Description

The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Specifications

• Antigen: CD3
• Clone: 1F4
• Conjugate: Brilliant Violet 421
• Format: Affinity Purified
• Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Isotype: IgM, κ
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Rat