CD3 Mouse anti-Rat, Brilliant Violet 421, Clone: 1F4, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 563948
The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|Brilliant Violet 421|
|F344 rat splenic T cells stimulated with PMA plus A23187|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
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