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CD3 Mouse anti-Rat, Brilliant Violet 605, Clone: 1F4, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563949

Catalog No. BDB563949


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Description

Description

The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and Cy™3.5. Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

Specifications

CD3
1F4
Brilliant Violet 605
Affinity Purified
F344 rat splenic T cells stimulated with PMA plus A23187
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Murine
IgM, κ
RUO
Primary
Rat
Documents
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