CD3 Mouse anti-Rat, Brilliant Violet 605, Clone: 1F4, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563949

Catalog No. BDB563949

Description

The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and Cy™3.5. Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: CD3
• Clone: 1F4
• Conjugate: Brilliant Violet 605
• Format: Affinity Purified
• Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Murine
• Isotype: IgM, κ
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Rat