CD3 Mouse anti-Human, NA/LE, Unlabeled, Clone: HIT3A, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 555336

Catalog No. BDB555336

Description

Reacts with the human ε-chain, a 20kDa subunit of CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjuction with pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. HIT3a antibody does not stain intracellular CD3 unlike the other CD3 clone, UCHT1. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD3
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: HIT3A
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG2a κ

For Research Use Only.