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Description
Reacts with the human ε-chain, a 20kDa subunit of CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjuction with pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. HIT3a antibody does not stain intracellular CD3 unlike the other CD3 clone, UCHT1. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Specifications
Specifications
| Antigen | CD3 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | HIT3A |
| Concentration | 1.0mg/mL |
| Conjugate | Unconjugated |
| Formulation | No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay. |
| Host Species | Mouse |
| Purification Method | Affinity Purified |
| Quantity | 0.5 mg |
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For Research Use Only.
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