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  6. CD34 Rat anti-Mouse, Alexa Fluor 647, Clone: RAM34, BD

CD34 Rat anti-Mouse, Alexa Fluor 647, Clone: RAM34, BD

Catalog No. BDB560230 Shop All BD Biosciences Products
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25 μg
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BDB560230 0.1 mg
BDB560233 25 μg
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Catalog No. BDB560230 Supplier BD Biosciences Supplier No. 560230
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Description

Rat Monoclonal Antibody

The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E
(Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.

Host Species: Rat
Clone: RAM34
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: Recombinant Mouse CD34

Flow Cytometry

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD34
Applications Flow Cytometry
Classification Monoclonal
Clone RAM34
Concentration 0.2mg/mL
Conjugate Alexa Fluor 647
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen Recombinant Mouse CD34
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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For Research Use Only.

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