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CD4 Mouse anti-Human, BUV395, Clone: L200, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 564107

Catalog No. BDB564107


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Description

Description

The L200 monoclonal antibody specifically binds to the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. The L200 antibody also crossreacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both Rhesus and Cynomolgus Macaque monkeys. Crossreactivity on both lymphocytes and monocytes (weak) from Baboons is also observed. The distribution on lymphocytes is similar for both human and monkey cells, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

Specifications

CD4
L200
L3T4 ; T-cell surface antigen T4/Leu-3; W3/25 ; CD4 antigen (p55)
Affinity Purified
Human HPB-ALL Cell Line
50 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
Brilliant Ultraviolet 395
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Murine
IgG1, κ
RUO
Primary
Baboon, Cynomolgus, Rhesus
SDS
Documents
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