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CD4 Rat anti-Mouse, BD IMag-DM, Clone: GK1.5, BD

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Description

Rat Monoclonal Antibody

BD IMag™ anti-mouse CD4 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD4-bearing leukocytes using the BD IMagnet™. The CD4 (L3T4) differentiation antigen has been reported to be expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells.

Leukocytes are labeled with BD IMag™ anti-mouse CD4 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat.No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD4
Applications Flow Cytometry
Classification Monoclonal
Clone GK1.5
Conjugate BD IMag-DM
Description Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Rat
Immunogen Mouse CTL clone V4
Purification Method Affinity Purified
Quantity 10 mL
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C
Isotype IgG2b κ
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For Research Use Only.

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