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CD45 Rat anti-Mouse, BUV395, Clone: 30-F11, BD

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BDB564279 50 μg
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Catalog No. BDB564279 Supplier BD Biosciences Supplier No. 564279
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Description

Rat Monoclonal Antibody

The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348 nmand an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45
Applications Flow Cytometry
Classification Monoclonal
Clone 30-F11
Concentration 0.2mg/mL
Conjugate Brilliant Ultraviolet 395
Description Ptprc; CD45R; LCA; Leukocyte common antigen; T200; B220; Ly-5; Lyt-4
Host Species Rat
Immunogen Mouse Thymus / Spleen
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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