CD69 Hamster anti-Mouse, APC-Cy7, Clone: H1.2F3, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 561240

Catalog No. BDB561240

Description

The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells.

Specifications

• Antigen: CD69
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: VEA; Very Early Activation Antigen; AIM; Activation Induced Molecule
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 λ3

• Applications: Flow Cytometry
• Clone: H1.2F3
• Conjugate: APC-Cyanine7
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Mouse Dendritic Epidermal T Cell Line Y245
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.