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Description
The AD2 monoclonal antibody specifically binds to ecto-5'-nucleotidase, a 70 kDa, glycosyl phosphatidylinositol (GPI)-anchored glycoprotein. CD73 is expressed on subsets of T and B lymphocytes, follicular dendritic cells, epithelial cells, endothelial cells and mesenchymal stem cells. Its expression on lymphocytes increases during T and B cell development. CD73 has enzymatic activity and catalyzes the dephosphorylation of adenosine monophosphate (AMP) converting it to adenosine. It has been suggested that CD73 can mediate costimulatory signals in T cell activation and adhesion of lymphocytes to endothelium. The antibody was conjugated to Brilliant Violet 421 and has been developed in collaboration with Sirigen. With an Ex Max of 407-nm and Em Max at 421-nm, Brilliant Violet 421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). Brilliant Violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Host Species: Mouse
Clone: AD2
Isotype: IgG1, κ
Species Reactivity: Human
Flow Cytometry
Specifications
Specifications
| Antigen | CD73 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | AD2 |
| Conjugate | Brilliant Violet 421 |
| Description | NT5E; 5' nucleotidase; 5'-NT; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5 |
| Formulation | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Pre-B leukemia cell line |
| Purification Method | Affinity Purified |
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Safety and Handling
For Research Use Only.
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