anti-CHEK2 Matched Antibody Pair, Abnova

Catalog No.	Quantity
89-017-410	1 Set

Catalog No. 89-017-410 Supplier Abnova Corporation Supplier No. H00011200AP11

Description

Includes

Rabbit MaxPab™ affinity purified polyclonal anti-CHEK2 (100μg); Mouse monoclonal anti-CHEK2, IgG2aκ (20μg); Sufficient for at least 3-5 x 96 well plates using recommended protocols.

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

ELISA

Specifications

• Antigen: CHEK2
• Applications: ELISA
• Classification: Monoclonal/Polyclonal
• Conjugate: Unconjugated
• Description: This antibody pair set comes with matched antibody pair to detect and quantify protein level of human CHEK2.
• Gene: CHEK2
• Gene Alias: CDS1/CHK2/HuCds1/LFS2/PP1425/RAD53
• Gene Symbols: CHEK2
• Host Species: Mouse, Rabbit
• Purification Method: Affinity Purified
• Quantity: 1 Set
• Regulatory Status: RUO
• Research Discipline: Cell Cycle
• Primary or Secondary: Primary
• Gene ID (Entrez): 11200
• Includes: Antibody pair set content: 1. Capture antibody: rabbit MaxPab™ affinity purified polyclonal anti-CHEK2 (100 μg) 2. Detection antibody: mouse monoclonal anti-CHEK2, IgG2a Kappa (20 μg) *Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.
• Target Species: Human
• Content And Storage: Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
• Isotype: IgG2a