Complement C4 Rat anti-Mouse, Clone: 16D2, Invitrogen™

Rat Monoclonal Antibody

Manufacturer:  Invitrogen MA140047

Catalog No. 01-676-500

Description

MA1-40047 detects C4 from mouse samples. MA1-40047 has been successfully used in immunohistochemistry (frozen), immunoprecipitation, ELISA, immunofluorescence and Western blot applications. The MA1-40047 immunogen is mouse complement factor C4. Suggested positive Control: T-cell regions of Mouse spleen.

Murine complement factor C4, formerly known as Gg protein, consists of alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway coverage at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a, and C4b. The latter can be cleaved to result in C4c and C4d in which step all functional sites are lost. The monoclonal antibody 16D2 reacts with intact C4, C4b, and C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes, and intestinal epithelial cells.

Specifications

• Antigen: Complement C4
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and 0.02% sodium azide
• Format: Liquid
• Gene Symbols: C4A
• Immunogen: Thymocytes decorated with Thy-1 antibody and complement components
• Purification Method: Protein G
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: ELISA, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: 16D2
• Conjugate: Unconjugated
• Gene: C4a
• Gene Alias: Complement component 4
• Host Species: Rat
• Isotype: IgG2a
• Quantity: 100 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 625018