Invitrogen™ FOXO1 Monoclonal Antibody (3B6)

Catalog No.	Quantity
PIMA517078	100 μg

Catalog No. PIMA517078 Supplier Invitrogen™ Supplier No. MA517078

Description

Mouse Monoclonal Antibody

MA5-17078 targets FOXO1 in ChIP, IHC, IF, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17078 immunogen is purified recombinant fragment of human FOXO1 expressed in E. Coli. MA5-17078 detects FOXO1 which has a predicted molecular weight of approximately 69.7kDa.

FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.

Specifications

• Antigen: FOXO1
• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 3B6
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: FOXO1
• Gene Accession No.: Q12778, Q9R1E0
• Gene Alias: Afxh; AI876417; FKH1; FKHR; Fkhr1; forkhead box O1; forkhead box O1 a; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); forkhead box protein O1; forkhead box protein O1; LOW QUALITY PROTEIN: forkhead box protein O1; forkhead box protein O1A; Forkhead box protein O1-A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead protein FKHR; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; fox family transcription factor FoxO1a.1; FOX01A; foxhead box protein O1-A; foxo1; FOXO1A; FoxO1a.1; member of FKHR-subclass forkhead/winged helix family; zgc:153388
• Gene Symbols: FOXO1
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human FOXO1 expressed in E. Coli.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2308, 56458
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1