Granzyme B Mouse anti-Human, V450, Clone: GB11, BD

Catalog No.	Quantity
BDB561151	50 Tests

Catalog No. BDB561151 Supplier BD Biosciences Supplier No. 561151

Description

Mouse Monoclonal Antibody

The GB11 antibody specifically reacts with human granzyme B, a serine protease ofapproximately 32 kDa. Granzyme B is stored in the granules of cytotoxic T lymphocytes and NK cells along with the pore-forming protein perforin. In the classic model of target cell lysis, perforins create holes in the target cell membrane allowing entrance of granzymes. Granzyme B has been shown to act on specific substrates including caspase-3, -7, -9, and -10 which in turn give rise to enzymes that mediate apoptosis. Granzyme B may also be involved in the hydrolysis of extracellular matrix components. Detectable levels of granzyme B have been detected in sera from healthy volunteers. The immunogen used to generate the GB11 hybridoma was human granzyme B isolated from an NK cell line.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Specifications

• Antigen: Granzyme B
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: GB11
• Conjugate: Violet Fluor 450 (V450)
• Description: GZMB; Granzyme-2; CCPI; CGL1; CSPB; CTLA1; CTSGL1; GRB; HLP; SECT
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Granzyme B
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov