MilliporeSigma™ anti-HNF-1-beta Clone: 12A5.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABE971

Catalog No. MABE971MI

Description

Specifically detects HNF-1-beta Clone: 12A5.1 in Human, Rat samples, and it is validated for Immunohistochemistry, Western Blotting

Hepatocyte nuclear factor 1-beta (UniProt P35680; also known as HNF-1-beta, HNF-1B, Homeoprotein LFB3, TCF-2, Transcription factor 2, Variant hepatic nuclear factor 1, vHNF1) is encoded by the HNF1B (also known as TCF2, VHNF1) gene (Gene ID 6928) in human. HNF-1-beta is involved in transcriptional and functional regulation of the liver and biliary system, as well as of the kidneys, urogenital tract and pancreatic β-cells. HNF-1-beta deficiency due to HNF1B mutations is a well known cause of maturity-onset diabetes of the young 5 (MODY5), also referred to as RCAD (renal cysts and diabetes) due to the frequent co-occurrence of renal cysts and/or other inborn urogenital abnormalities. HNF-1-beta is expressed in the gall bladder, liver and intrahepatic bile ducts of mouse embryos and in liver of adult mice. HNF-1-beta is shown to co-regulate morphogenesis of the biliary system and HNF1B knockout mice suffer from severe neonatal cholestasis and jaundice due to the abnormally developed gall bladder and dysfunctional intrahepatic bile ducts.

Specifications

• Antigen: HNF-1-beta
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: P35680
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Expected to react with isoform 1 (A) and isoform 3 (C). Reactivity toward isoform 2 (B) and isoform 4 has not been determined.
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG2a κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 12A5.1
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: HNF1B, TCF2
• Immunogen: GST-tagged recombinant protein corresponding to the Internal (N-terminal half) of human HNF-1-beta.
• Quantity: 100 μg
• Research Discipline: Epigenetics, Nuclear Function
• Gene ID (Entrez): NP_000449
• Target Species: Human, Rat
• Form: Purified

For Research Use Only