Huntingtin Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1004
Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site. Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody PA1-004 recognizes the 586 cleaved fragment without detecting the full-length form.Huntingtin is a disease gene linked to Huntington ft.s disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predomitly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington ft.s disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
|Synthetic peptide conjugated to KLH via cysteine corresponding to CPSDSSEIVLD (577-586) of Human HTT.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Htt, Huntingtin, HD, IT15|
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