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CD25 Mouse anti-Human, Brilliant Violet 605, Clone: 2A3, BD

Mouse Monoclonal Antibody

$220.00 - $395.00

Specifications

Antigen CD25
Isotype IgG1, κ
Immunogen Human PHA-activated T Cells
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Conjugate Brilliant Violet 605
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB562661
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BD Biosciences
562661
25 Tests Each for $220.00
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BDB562660
View Documents
BD Biosciences
562660
100 Tests Each for $395.00
Add to cart
 
Description

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL -2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

Specifications

CD25
Human PHA-activated T Cells
Brilliant Violet 605
Flow Cytometry
RUO
2A3
IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
IgG1, κ
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Primary
Murine
Affinity Purified
Monoclonal
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.

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